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Biology Coursework

The Effect of Different concentrations of Aspirin on the Antibiotics on Escherichia coli

This investigation mainly looks at the effects of aspirin (acetyl-salicylic acid) on the antibiotics resistance of the bacterium Escherichia coli. Through thorough trialing, the investigation then focused on the effect of varying concentrations of aspirin on the antibiotic on the growth of Escherichia coli(E coli) on agar plates. Aspirin is an acetyl derivative of salicylic acid, called acetylsalicylic acid. It is a major analgesic and antipyretic to relief pain and fever. It can also be used to reduce heart disease and strokes. Using Aseptic technique, I spread the E. coli on agar plates, and inserted 1 filter paper disk with 1 antibiotic disk per plate saturated with varying concentration of aspirin. Later, I incubated then and then measure the area of inhibition. The result shows that as the concentration of the aspirin increases, the area of clearance decreases accordingly.


Aspirin, acetylsalicylic acid belongs to the chemicals called salicylates. Salicylic acid, the main metabolite of aspirin, plays a significant role in human and animal metabolism.   Aspirin works by inhibitng the pain enhanceing enzyme cyclooxygenase 2. It suppresses the action of the enzyme COX that produces prostaglandins. Prostaglandins are among the chemicals secreted by the body’s immune system when it fights off bacteria and other invaders in injuries and these chemicals use an enzyme called cyclooxygenae 2 for the cells in the damaged tissues, This will also cause pain and inflammation.   Aspirin can disrupt the pathway and avoid pain, inflammation, elevated temperature, and stomach protection. Aspirin can lead to irreversible inactivation of the cyclooxygenase, which is required for prostaglandin and thromboxane synthesis, thus can then stop the cells from making prostaglandin and...

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