Anti Essays offers essay examples to help students with their essay writing.

Sign Up

Observations of Cell Size Dynamics Under Osmotic Stress Essay

Open Document

Below is an essay on "Observations of Cell Size Dynamics Under Osmotic Stress" from Anti Essays, your source for research papers, essays, and term paper examples.

Brief Report

Observations of Cell Size Dynamics Under Osmotic Stress
Thomas R. Kiehl,1 Duan Shen,2 Sarwat F. Khattak,3 Zheng Jian Li,3 Susan T. Sharfstein2*
 Abstract Cultured mammalian cells [e.g., murine hybridomas, Chinese hamster ovary (CHO) cells] used to produce therapeutic and diagnostic proteins often exhibit increased specific productivity under osmotic stress. This increase in specific productivity is accompanied by a number of physiological changes, including cell size variation. Investigating the cell size variation of hyperosmotically stressed cultures may reveal, in part, the basis for increased specific productivity as well as an understanding of some of the cellular defense responses that occur under hyperosmotic conditions. The regulation of cell volume is a critical function maintained in animal cells. Although these cells are highly permeable to water, they are significantly less permeable to ionic solutes. Appropriate cell– water content is actively maintained in these cells by regulation of ion and osmolyte balances. Transport appropriate to extracellular conditions, leading to accrual or release of these species, is activated in response to acute cell volume changes. Osmotically induced regulatory volume increases (RVI) and regulatory volume decreases (RVD) are known to occur under a variety of conditions. We observed the time evolution of size variation in populations of two CHO cell lines under hyperosmotic conditions. Observations were made using multiple instruments, multiple cell lines, and multiple cell culture conditions. Size variation of CHO A1 was gauged by flow cytometry using an LSRII1 flow cytometer while CHO B0 cells were quantified using a Cedex1 cell analyzer. Hyperosmotic stress had a dose-dependent effect on the regulatory control of cell volume. Stressed cultures of CHO cells grown in suspension exhibited a shift in mean cell diameter. This shift in mean was not due to a change in the whole population, but rather to...

Show More


MLA Citation

"Observations of Cell Size Dynamics Under Osmotic Stress". Anti Essays. 18 Dec. 2018


APA Citation

Observations of Cell Size Dynamics Under Osmotic Stress. Anti Essays. Retrieved December 18, 2018, from the World Wide Web: http://parimatch-stavka7.com/free-essays/Observations-Of-Cell-Size-Dynamics-Under-393132.html

Faith Hunter | Orphan black | Dragon Ball Z: Plan to Eradicate Saiyans Episode 2 English Subbed